Fuel Economy.Gov notes aggressive driving (rapid acceleration & braking) decreases your gas mileage by as much as 33%!
Keep it below 60mph
Every 5 mph over 60 reduces your gas mileage about 4 mpg or about 15% for most cars.
Remove Excessive Weight
Especially for the Smaller Vehicles. 100 extra pounds can reduce your mpg's about 2%
Avoid Excessive Idling
Idling costs about 5 cents per minute. It only takes a few seconds worth of fuel to restart your vehicle.
Use Cruise & Overdrive
Cruise control will help reduce the losses from aggressive driving, and Overdrive will reduce your engine speed once your vehicle reaches a crusing speed.